Table 2.
Quantification of IgE+ B cells and sequencing depth.
| % CD19+ | IgE+ B cells | VDJ sequences | Median theoretical | |||
|---|---|---|---|---|---|---|
| Donor | cells within live gate | % within CD19+ gate | per 2 × 106 CD19+ cells | Total | per cell | coverage (range) |
| 1 | 11.3 | 0.10 | 2,000 | 3,254 | 1.63 | 81% (79–82) |
| 2 | 10.8 | 0.09 | 1,800 | 1,814 | 1.01 | 63% (62–66) |
| 3 | 10.8 | 0.15 | 3,000 | 3,716 | 1.24 | 71% (69–73) |
| 4 | 9.2 | 0.06 | 1,200 | 1,823 | 1.52 | 78% (76–81) |
| 5 | 6.5 | 0.08 | 1,600 | 5,199 | 3.25 | 96% (95–97) |
| Median | 10.8 | 0.09 | 2,000 | 3,254 | 1.52 | 78% |
Coverage represents the amount of unique sequences sampled from the pool in an in silico simulation assuming no PCR amplification bias. Its range is based on the minimum and maximum values obtained from 100 simulations.