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Characterisation of CPC cells. A) CPCs under phase contrast microscope, as observed at (a) p2, (b) p4, and (c) p7. (scale bars 100 μm). B) Gene expression levels of stemness and cardiac lineage markers in CPCs at P4, compared to mESCs differentiating to CMs at d4 and d7 of differentiation (n = 3). C) Immunocytochemistry on CPCs, (a-c) SCA1 (d-f), CKIT, (g-i) CD90/SCA1 double staining, with DAPI (a, d, g), (k) secondary antibodies for negative control. Scale bars: 50 μm.
