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AKT1/2 knockdown suppresses colony formation of ESCC cells. (a) AKT1/2 expression was knocked down in KYSE70, 450 and 510 ESCC cell lines. (b) Anchorage‐independent cell growth is decreased with or without xanthohumol treatment after AKT1/2 expression was decreased. (c) Representative colony pictures after AKT1/2 silenced with or without xanthohumol treatment. Asterisks (*p < 0.05, **p < 0.01, ***p < 0.001) indicate a significant decrease in colony formation of ESCC cells.
