Figure 7.

Alpha mannosidase treatment facilitates P. aeruginosa clearance in C57BL6/N mice. (A) P. aeruginosa 6294 was allowed to form biofilms in vitro for 24 h and were exposed to 10 U of α‐mannosidase for 2 h at 37°C. Bacterial biofilms stained and measured. Student's t test. (P = 0.006). Data are representative of two experiments. (B) P. aeruginosa PAO1‐CrdA‐GFP was allowed to form biofilms in vitro for 24 h and were exposed to 10 U of α‐mannosidase for 2 h at 37°C. Bacterial biofilms were washed, and fluorescent signal measured. Student's t test. (P = 0.05). Data are representative of two independent experiments. (C) C57BL/6N were infected with P. aeruginosa 6294 at 1.10⁶ CFU/cornea, treated with 0.2 U/drop α‐mannosidase four times daily, starting at 12 h post the onset of infection. Student's t test, (P = 0.01). Data are representative of two independent experiments with 5 mice per group