Figure 4.

Localisation of cyanobacterial proteins targeted to rice chloroplasts. Confocal microscopy images showing chlorophyll autofluorescence (“Autofluor.”), yellow fluorescent protein (YFP) signal, and the overlay of these two channels for carboxysome shell (a) and internal (b) YFP fusion proteins. “p1”–“p9” denote the different promoters used to drive expression of the various cyanobacterial transgenes. Additional examples of subcellular localisation of fluorescent proteins (c): to the chloroplast envelope using a chloroplast envelope transit peptide (ceTP), to the cytosol in the absence of a transit peptide, and the simultaneous localisation of two fluorescent proteins (DsRed and tGFP) to different compartments. All images are representative for each cassette. The same magnification (400×) was used to acquire all images, scale bars represent 10 μm