Skip to main content
. 2019 Jul 3;10:1535. doi: 10.3389/fimmu.2019.01535

Figure 1.

Figure 1

(A) pSTAT-3 expression was determined in true naïve (TN; CD62L+ CD45RA+), central memory (CM; CD62L+ CD45RA-), and effector memory (EM; CD62L- CD45RA-) CD4+ T-cells in peripheral blood of a previously described early arthritis patient cohort (3) using flow cytometry. (B) In the same cohort, the relationship between pSTAT-3 and paired circulating interleukin (IL)-6, measured by MSD immunoassay, was assessed by Spearman correlation coefficients (rho); gradients of best-fit lines differ significantly (analyses of covariance p < 0.001). Amongst newly-enrolled early arthritis patients (cohort A; see Table 1), surface IL-6R expression (C) and surface gp130 expression (D) was determined in TN, CM and EM CD4+ T-cells in the peripheral blood using flow cytometry [left panels; n = 26; **p < 0.001, ***p < 0.0001, non-parametric analysis of variance (Friedman's) with Dunn's post hoc pairwise analyses; associated p-value are depicted]. Exemplar FACS plots are also shown (right panels).