Figure 2.

Naïve or memory CD4+ T cells were isolated from 3 healthy donors and cultured with 0.5 ng/ml IL-6 and equimolar sIL-6R for 72 h prior to washing and stimulation with 0.2 μg/ml anti-CD3 and 1 μg/ml anti-CD28 for 4 h. RNA was extracted at baseline (t0), after 6 or 72 h exposure to IL-6 (t1, t2) and 4 h post-TCR stimulation (t3) as depicted by the red arrows.