Figure 3.

CHAC1 overexpression in proton pump/H,K‐ATPase α subunit (PUMP)‐positive parietal cells with Helicobacter.pylori (HP) infection. Double‐enzyme or fluorescence IHC for an identical sample of H. pylori‐infected fundic‐gland mucosa from a subject with chronic atrophic gastritis. H. pylori is located in the proton pump‐positive cells (arrows), (A and B) with anti‐proton pump antibody blue or green and TMDU‐mAb (anti‐H. pylori) brown or red. CHAC1 is overexpressed in the proton pump‐positive cells (arrows) (C and D) with CHAC1‐mAb blue or green and anti‐proton pump antibody brown or red. CHAC1 is overexpressed in parietal cells with H. pylori infection (arrows) (E and F) with CHAC1‐mAb blue or green and TMDU‐mAb brown or red. Bars: 50 µm