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. 2019 Apr 29;70(1):259–275. doi: 10.1002/hep.30613

Figure 3.

Figure 3

Silencing lnc‐UCID post‐transcriptionally down‐regulates CDK6 expression. (A) Lnc‐UCID knockdown reduced the level of phosphorylated pRb. (B,C) Lnc‐UCID knockdown reduced the protein and mRNA levels of CDK6. Forty‐eight hours after transfection with NC or siUCID, the protein (A,B) and mRNA (C) levels of genes were assessed by western blotting and quantitative real‐time PCR, respectively. (D) Lnc‐UCID knockdown reduced the stability of CDK6 mRNA. NC‐transfected or siUCID‐transfected cells were treated with 5 μg/mL actinomycin D for the indicated times. (E) Lnc‐UCID knockdown reduced the proportion of CDK6 mRNA in the polysome fraction. For (C) and (E), error bars represent mean ± SEM from three independent experiments. (F) CDK6 levels were positively correlated with lnc‐UCID levels. The RNA levels of CDK6 and lnc‐UCID were detected in 62 paired HCC and nontumor liver tissues by quantitative real‐time PCR. *P < 0.05; **P < 0.01; ***P < 0.001. Abbreviation: ppRb, S780‐phosphorylated pRb.