Table 1.
Fraction | Volume (mL) | Activity (AU mL‐1) | Total activity (AU) | Protein content (mg mL−1) | Specific activity (AU mg‐1 protein) | Yield (%) |
---|---|---|---|---|---|---|
Culture medium | 7000 | 337 | 2 359 000 | n.d.a | n.d. | 100 |
Supernatant | 6300 | 333 | 2 097 900 | 6.0 | 56 | 90 |
After ammonium sulfate precipitation, 10× redissolved | 630 | 933 | 587 790 | 3.2 | 291 | 28 |
Sakacin‐A extract | 31.60 g | 16.70 AU mg‐1 | 527 720 | 63 mg g−1 | 265 | 25 |
n.d., not determinable.