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. 2018 Sep 12;11(4):487–494. doi: 10.1111/1758-2229.12684

Figure 1.

Figure 1

Details and relative performance of primers used in this study for identification of Archaea.

A. Map of small ribosomal RNA subunit (SSU) and primers used in this study. Primers designed in this study are indicated in italic.

B–D. The performance of tested primer pairs (sorted by the proportion of Archaea sequences) for amplification of archaeal 16S rRNA genes: (B) the proportion of Archaea sequences across samples, (C) relative OTU richness in relation to average richness in each sample, and (D) relative phylum richness in relation to average richness in each sample. The solid line indicated average level among samples. Different letters denote significant differences (P < 0.05). Error bars show standard error. Note that several primer pairs were excluded from B–D due to their performance in amplifying Archaea. For the same reason, the reference primer pair 515f + 806rB was excluded from C and D. S, short amplicons (< 700 bp); L, long amplicons (> 700 bp); * recommended based on this study.