Figure 2.

Astrocyte‐specific Afg3l2 deletion causes BG degenerative changes. (a) Immunostaining of BG using S100, EAAT1, or EAAT2 antibodies at different time‐points (n at least three for each group at each time‐point). Scale bar: 20 μm. Arrows indicate displaced BG cell bodies in ML. (b) Dot plots showing quantification of S100⁺ cell bodies in the PCL and ML at different ages (mean ± SEM is indicated). (c) Distribution of S100⁺ cells in the PCL and ML at different ages (n is indicated in each column). p values were obtained by Student's t test. BG, Bergmann glia; GCL, granule cell layer; ML, molecular layer; PCL, PC layer [Color figure can be viewed at wileyonlinelibrary.com]