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. 2019 Apr 11;248(3):377–389. doi: 10.1002/path.5267

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© 2019 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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PATH-5267-FIG-0002-c — Renal cancer stem cells are characterized by IL‐8 and CXCR1 expression. (A) Heatmap depicting differential gene expression in the spheres compared to the corresponding parental cells using the Qiagen human CSC gene expression array. (B) CyTOF analysis of cellular expression of CD13, CD10, E‐cadherin, CD24, CD44, and CXCR4 in Caki‐1 spheres and parental cells. (C) Differential gene expression analyzed by RT‐qPCR for IL8 and CXCR1 in spheres compared to parental cells for 769P, A498, Caki‐1, and ACHN (one‐way ANOVA, n = 3). (D) Western blot for IL‐8 and CXCR1 in spheres and parental cells derived from ccRCC cell lines.