Figure 3.

The effect of systemic pregabalin on WDR neuronal responses in MIA and sham animals. (a) Following a subcutaneous injection of pregabalin (10 mg/kg), there was no neuronal inhibition induced by DNIC, yet there was a significant reduction in pre‐conditioned neuronal firing for all mechanical stimulations tested (n = 6). (b) Pregabalin had little effect on the degree of neuronal inhibition induced by DNIC in sham controls (n = 6) and had no statistically significant effect on mechanically evoked pre‐conditioned neuronal responses. (c–d) There was no significant difference in the mRNA expression levels of the α2δ1 subunit in either ipsilateral lumbar DRGs or dorsal horn. Two‐way ANOVA with Bonferroni correction tested statistically significant differences from pre‐conditioned baseline responses for both DNIC‐induced and drug‐induced neuronal inhibition, independent samples t test tested statistically significant differences in mRNA expression levels; *p < 0.05, **p < 0.01, ***p < 0.001