Figure 3.

LR34 transports abscisic acid (ABA). (a) Rice blast resistance and leaf tip necrosis in 4‐wk‐old plants of Lr34res‐expressing line 19, Lr34sus‐expressing line 131 and 19sib. (b) Tritiated abscisic acid (³H‐ABA) accumulation (disintegrations per minute, d.p.m.) in rice seedlings of lines 11, 19, and 131 compared with respective sib lines. N = 3, n = 13–23, mean ± SEM. *, P < 0.01 compared with sib (Student's t‐test). (c) Relative ³H‐ABA accumulation in yeast strain W303 expressing Lr34res or Lr34sus. Mean ± SE, **, P < 0.01 compared with empty vector (EV) control. The graphs show averaged results of three independent experiments. (d) Relative ³H‐ABA accumulation in yeast strain YMM12 expressing Lr34res or Lr34sus. Mean ± SE, **, P < 0.01 compared with EV control. The graphs show averaged results of three independent experiments. (e) Relative ³H‐ABA accumulation in yeast strain YMM12 after preincubation with the ABC transporter inhibitor glibenclamide (GC) and the ABCC‐specific inhibitor probenecid (Pro). The control (Con) without inhibitor was set to 100%, mean ± SE. *, P < 0.05, **, P < 0.01. (f) Relative ³H‐ABA accumulation (50 nM) in yeast strain YMM12 in the absence (Con) or presence of cold ABA. The control (Con) without inhibitor was set to 100%, mean ± SEM. *, P < 0.05, **, P < 0.01.