Figure 4.

RedIL6 fluorescence is present in the central amygdala (CeA) and i.c.v. administration of exendin‐4 (Ex‐4) increased interleukin (IL)‐6 mRNA expression in the CeA. A, B, Cells in the CeA that contain IL‐6, as indicated by RedIL6 reporter mouse (red), after i.p. infusion of vehicle. Shown is a representative overview of the CeA (A) and magnification (B) of the indicated parts of the CeA in (A). Red arrowheads indicate examples of IL‐6 fluorescent cells (B). Scale bars: overview = 80 μm, zoom = 10 μm. C, The results of a reverse transcriptase‐polymerase chain reaction from 8 + 8 mice showed a 1.5‐fold increase in IL‐6 mRNA after i.c.v. infusion of Ex‐4 compared to infusion of vehicle (artificial cerebrospinal fluid). *P < 0.05 and **P < 0.01, respectively