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. 2019 May 23;31(6):e12722. doi: 10.1111/jne.12722

Figure 5.

Figure 5

IL‐6 receptor‐alpha (IL‐6Rα) was present in NeuN‐positive cells in the central amygdala and IL‐6 stimulated the firing rate of neurones residing in the capsular part of the central amygdala. IL‐6Rα immunoreactivity (green) and NeuN immunoreactivity (red) partially co‐localised in the central amygdala (CeA). Cell nuclei were stained with 4′,6‐diamidino‐2‐phenylindole (blue). Yellow arrowheads show examples of cells where IL‐6Rα and NeuN immunoreactivity co‐localised, whereas green and red arrowheads show examples of cells with only IL‐6Rα and NeuN immunoreactivity, respectively (A, B). Approximately 50% of IL‐6Rα‐immunoreactive cells also showed NeuN immunoreactivity. Conversely, approximately 50% of NeuN immunoreactive cells also showed IL‐6Rα immunoreactivity (C, D). These results indicate that a substantial proportion of the neurones in the CeA could be responsive to IL‐6. Loose‐patch recording showed that IL‐6 (1 nmol L‐1) could rapidly increase the firing rate of the targeted neurones. The zoomed periods and frequency distribution graph under the recording also show this profound elevation (E). A cocktail of IL‐6 and its neutralising antibody (IL‐6 ab) evoked no significant change in the firing rate (F). Arrows show the onset of administration of IL‐6 or the IL‐6 + IL‐6ab cocktail. Scale bars: overview = 80 μm, zoom = 10 μm. BLA, basolateral amygdala