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. 2019 Apr 11;69(6):2546–2561. doi: 10.1002/hep.30571

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© 2019 The Authors. Hepatology published by Wiley Periodicals, Inc., on behalf of American Association for the Study of Liver Diseases.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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HBx influences CRL4^WDR70‐dependent end resection through its H‐box. (A) Immunoprecipitation of endogenous DDB1 by Flag‐WDR70 in the presence or not of overexpressed HBx. Flag‐WDR70 plasmid was transfected into L02 cells 24 hours before infection by pLV‐HA‐HBx lentivirus. Cells were lysed for coimmunoprecipitation analysis 48 hours after infection. α‐WDR70 and α‐DDB1 were used to detected individual proteins. (B) Immunoprecipitation of endogenous DDB1 by Flag‐WDR70 in L02 and T43 cells. (C) Rescue of DDB1–WDR70 interaction in T43 cells by introducing siHBx. (D) Restoration of IR‐induced pRPA32 by forced expression of DDB1 or Flag‐WDR70 in HBx‐expressing L02 cells. Note that the protein level of HBx is elevated upon DDB1 overexpression, possibly due to a stabilizing effect of their interaction.29 (E,F) Decreased RPA32 phosphorylation (E) and interaction between endogenous Flag‐WDR70 and DDB1 (F) in L02 cells in the presence of HA‐tagged SV5‐V serving as an H‐box surrogate for HBx. Abbreviations: HA, hemagglutinin; IP, immunoprecipitation.