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. 2019 Apr 11;69(6):2546–2561. doi: 10.1002/hep.30571

Figure 7.

Figure 7

Loss of H2B monoubiquitylation promotes tumorigenesis. (A) Subcutaneous xenograft of L02 cells preinfected by indicated lentivirus. Note the suppression of tumorigenesis of 2KR by coexpression of DDB1 or WDR70. (B) Immunoblotting for H2B monoubiquitylation in L02 cells by overexpression of human USP22 (left). Cells expressing USP22 were inoculated in nude mice and measured for tumor formation (right). (C) Xenograft tumor formation assay in nude mice. Individual tumor samples were dissected from nude nice 10 days after subcutaneous injection of L02 or T43 cells (left), and average tumor weights (right) were calculated. n = 9 dissected samples. Error bars, SD. **P < 0.01. (D) As in (C), tumor formation assays for T43 cells preinfected with pLV‐vector, pLV‐DDB1, or pLV‐WDR70 lentivirus. (E) IHC images (inset) and quantification of uH2B positivity for paraffin‐embedded xenografts. Nuclei were counterstained by hematoxylin (blue). n = 10 dissected samples. Abbreviation: LV, lentivirus.