Figure 1.

Generation and validation of the targeting GNrep construct. (a) GN reporter cassette was generated by subcloning the nucleus and the Golgi complex reporter sequences into the pUC57‐2A plasmid. This plasmid codes for the GTS‐mCherry and the H2B‐GFP sequences. The pUC57‐GNrep was used to generate a pME‐GNrep and subsequently the pEF1‐GNrep plasmid, by Gateway cloning, using the pEF1‐DEST as a destination vector. (b) NIH/3T3 fibroblasts and human umbilical vein endothelial cells transfected with pEF1‐GNrep plasmid revealed efficient expression of the GN reporter cassette. Nuclei are labeled with DAPI. Golgi apparatus is stained for GOLPH4 in NIH/3T3 cells. Scale bar, 10 μm