Figure 2.

Inhibition of X inactivate‐specific transcript (XIST) alleviated cell apoptosis and inflammation injuries in lipopolysaccharide (LPS)‐induced WI‐38 cells. A, XIST expression was detected by qRT‐PCR. B, The transfected efficiency of si‐XIST was analysed by qRT‐PCR. C, The effect of XIST knockdown on cell viability was analysed by CCK‐8 assay. The effect of XIST knockdown on cell apoptosis was evaluated by (D) flow cytometry and (E) Western blotting. The expression levels of IL‐6, IL‐1β, and TNF‐α were analysed by (F) ELISA, (G) qRT‐PCR, and (H) Western blotting. *P < .05, **P < .01, and ***P < .001