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. 2019 Jul 3;10:1536. doi: 10.3389/fimmu.2019.01536

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Copyright © 2019 Kanmani and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of TLRs negative regulators expression in HCT116 cells. After HCT116 cells treated LAB strains and PolyI:C for 3 and 12 h. The expression of A20, Tollip, SIGIRR, and IRAK-M were evaluated by RT-PCR. Cells treated with either PolyI:C or medium alone were used as positive and negative controls, respectively. The positive control was used for comparison of LAB strains treated groups The mean differences among the different superscript letters (a, b, ab) were significant at 0.05 level. LABs were able to modulate expression of negative regulators of TLR signaling in HCT116 cells, stimulated with PolyI:C.