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. 2019 Jul 3;10:1519. doi: 10.3389/fimmu.2019.01519

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Copyright © 2019 Ning, Wang, Zhou, Lu, Kang, Ding, Shen, Xu and Bai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Specific anti-DisA antibodies in DisA immunized mice, TB patients, and Mtb-infected mice. (A) Sera were collected from purified protein DisA immunized BALB/c mice in our previous study and sera from PBS immunized mice as control, antibody titers against DisA were assayed by ELISA as shown dilution. (B) Antibody titers against DisA in TB patients (n = 53) and healthy control (n = 113) were assayed by ELISA (1:200). (C) Antibody titers against DisA in Mtb-infected mice. Sera were collected from BALB/c mice monthly at 1–6 month (m) post-infection, and specific anti-DisA antibody titers were detected by ELISA (1:200).