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. 2019 Jul 3;10:1519. doi: 10.3389/fimmu.2019.01519

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Copyright © 2019 Ning, Wang, Zhou, Lu, Kang, Ding, Shen, Xu and Bai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of rBCG. (A) DisA was examined in BCG and rBCG using Western Blot analysis with anti-DisA polyclonal antibody. (B) Determination of intracellular c-di-AMP concentrations of the BCG and rBCG-DisA strains by HPLC. (C) Bacterial growth curve of the BCG and rBCG-disA strains. The growth was monitored using a microplate reader at 600 nm from day 1 to day 9 at 24-h intervals. (D) Morphology of the BCG and rBCG-DisA strains stained with Ziehl-Neelsen acid-fast staining and observed under a light microscope (1 000×). (E) Morphology of BCG and rBCG-DisA strains observed by transmission electron microscope (4 200×), and bacterial cell sizes (n = 100) were measured by Image J software. ^*P < 0.05, ^***P < 0.001.