Table 1.
Identification of Calcineurin subunits by MALDI-TOF mass spectrometrya
| Peptide | Start | End | M/z (Obs) | Mr (Exp) | Mr (Calc) | δ | Missed cleavage | Sequence | Modification |
|---|---|---|---|---|---|---|---|---|---|
| CaNA (isoform α): 11 peptides; protein score: 132; protein mass: 59,291 Da; peptide coverage: 25% | |||||||||
| 1 | 143 | 148 | 762.49 | 761.4786 | 761.4799 | −0.001 | 0 | K.TLFLLR | |
| 2 | 20 | 28 | 1007.55 | 1006.5437 | 1006.5348 | 0.009 | 0 | K.AVPFPPSHR | |
| 3 | 64 | 73 | 1072.65 | 1071.6405 | 1071.6288 | 0.012 | 0 | R.IITEGASILR | |
| 4 | 280 | 289 | 1117.55 | 1116.5398 | 1116.4948 | 0.045 | 0 | R.AHEAQDAGYR | |
| 5 | 324 | 332 | 1152.55 | 1151.5477 | 1151.5393 | 0.008 | 0 | K.YENNVMNIR | |
| 6 | 33 | 42 | 1176.58 | 1175.5759 | 1175.557 | 0.019 | 0 | K.EVFDNDGKPR | |
| 7 | 101 | 112 | 1247.64 | 1246.6361 | 1246.6305 | 0.006 | 0 | K.LFEVGGSPANTR | |
| 8 | 113 | 122 | 1260.64 | 1259.6346 | 1259.6186 | 0.016 | 0 | R.YLFLGDYVDR | |
| 9 | 244 | 254 | 1369.66 | 1368.6549 | 1368.6534 | 0.002 | 0 | K.TQEHFTHNTVR | |
| 10 | 488 | 501 | 1489.75 | 1488.7407 | 1488.6878 | 0.053 | 0 | R.DAMPSDANLNSINK | |
| 11 | 425 | 441 | 1571.89 | 1570.8787 | 1570.8388 | 0.040 | 0 | K.GLTPTGMLPSGVLSGGK | Oxidation (M) |
| CaNB (isoform 1): 5 peptides; protein score: 56; protein mass: 19,244 Da; peptide coverage: 35% | |||||||||
| 1 | 117 | 124 | 906.43 | 905.42 | 905.45 | −0.02 | 0 | K.MMVGNNLK | |
| 2 | 125 | 134 | 1187.65 | 1186.65 | 1186.62 | 0.03 | 0 | K.DTQLQQIVDK | |
| 3 | 135 | 146 | 1274.67 | 1273.66 | 1273.63 | 0.04 | 1 | K.TIINADKDGDGR | |
| 4 | 73 | 84 | 1369.73 | 1368.72 | 1368.69 | 0.03 | 0 | K.EFIEGVSQFSVK | |
| 5 | 57 | 72 | 1783.86 | 1782.85 | 1782.83 | 0.02 | 0 | R.VIDIFDTDGNGEVDFK |
aThe lists of peptides matching with the CaNA (isoform α) and CaNB (isoform 1) sequences are shown. For each peptide identified, the position in protein sequence, experimental and calculated masses, number of trypsin missed cleavage, and sequence are indicated.