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. 2013 Jun 12;33(24):10037–10047. doi: 10.1523/JNEUROSCI.3137-12.2013

Figure 5.

Figure 5.

Inflammatory reactions are altered in the corpus callosum in cuprizone-fed GnT-IX-deficient mice. A, Astrogliosis is decreased in GnT-IX-deficient mice. After 8 weeks of cuprizone treatment, brain sections from wild-type (WT) and GnT-IX-deficient (KO) mice were stained with Cat-315 (red) and anti-GFAP (green) antibodies. Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm. B, Microglia recruitment to the corpus callosum is increased in GnT-IX-deficient mice. Brain sections were stained with Cat-315 (red) and anti-Iba1 (green) antibodies to detect microglia. The enclosed areas indicate the borders of the corpus callosum. Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm. C, Astrogliosis in the corpus callosum was quantified by measuring the relative GFAP-positive areas. The data shown are means ± SEM (n = 9). *p < 0.05. D, Quantification of Iba1-positive cells in the corpus callosum. The data shown are means ± SEM (n = 6).