Figure 6.

Both the Cat-315 and RPTPβ epitopes are increased in activated astrocytes in the corpus callosum by cuprizone feeding. A, After 8 weeks of cuprizone treatment, brain sections from wild-type mice were stained with Cat-315 (green), anti-RPTPβ (green), anti-GFAP (red), and anti-Olig2 (red) antibodies. Scale bars, 20 μm. The enclosed areas are magnified on the right (Scale bars, 5 μm). B, A series of cell cultures that mainly contained astrocytes, neurons, or oligodendrocytes were prepared from wild-type mice, fixed, and stained with Cat-315 (red) and anti-GFAP, anti-MAP2, and anti-MBP (green) antibodies, respectively. Nuclei were counterstained with DAPI (blue). Scale bars, 20 μm.