Table 2.
Forward and reverse primers used for the qRT-PCR reaction
| Gene | Genebank reference no. | Primers | 5′–3′ | Apmlicon size |
|---|---|---|---|---|
| SNAP-25 (bovine) | NM_001076246.1 | Fw | GGCCGATGAGTCTCTGGAAA | 128 |
| Rev | TCCCTTCCTCAATGCGTTCC | |||
| SNAP-25 (mouse) | NM_011428.3 | Fw | GGATGAGCAAGGCGAACAAC | 180 |
| Rev | TCCTGATTATTGCCCCAGGC | |||
| Munc18-1 (bovine) | NM_174619.2 | Fw | GCTCATAGGATCCACGCACA | 110 |
| Rev | TGGAGGGGCGGCTTATTTTT | |||
| Munc18-2 (bovine) | NM_001046208.2 | Fw | CTACGGAATGGTGTGAGCGA | 189 |
| Rev | CCAGCGAGACAGCTGATAGG | |||
| Munc18-2 (human) | NM_006949.2 | Fw | ATCAACAAACGGCGGGAACC | 96 |
| Rev | GTCTTTGATCAGGGCCTTCTCC | |||
| Syt 1 (bovine) | NM_174192.3 | Fw | AGCTGGTGGCCCTGGAAA | 177 |
| Rev | CTGGTTTTCCTGGAGCGGG | |||
| Stx 1 (bovine) | NM_001083798.1 | Fw | GAGTTCTTCGAGCAGGTGGA | 180 |
| Rev | TTGGAGCGGACTTTGTTTGC | |||
| Stx 1 (rat) | NM_053788.2 | Fw | AGCGATGACGACGATGATGT | 178 |
| Rev | CCTTGGTCTTCTCATCGGGG | |||
| Rab3a (bovine) | NM_174446.3 | Fw | CCACAGCATCAGTGCAGAAC | 160 |
| Rev | GAGTCGTCTGCATAGCGGAA | |||
| Cplx 2 (bovine) | NM_174283.2 | Fw | CATCGCCCCCAAGCCAG | 123 |
| Rev | TTGGTGGCCCCTCCAAGG | |||
| UBC (bovine) | NM_001206307.1 | Fw | GGACCGGGAGTTCAGTCTTC | 100 |
| Rev | TGATGGTTTTACCAGTGAGGGT |
Syt, synaptotagmin; Stx, syntaxin ; Cplx, complexin.