Figure 7.

Impaired GABA transmission leads to defective sleep in dnlg4 mutant flies. A, Average sleep traces for wild-type (WT), dnlg4, Rdl^A302S, and Rdl^A302S,dnlg4 flies plotted as a 30 min moving average, n = 32. B, C, Quantification of total sleep time and sleep onset latency after lights off for each genotype; n = 32, *p < 0.001 for dnlg4 mutant versus Rdl^A302S,dnlg4 double mutant flies. D, Effect of different CBZ concentrations on the sleep pattern of WT (top) and dnlg4 mutant (bottom) flies during the first day of drug treatment: n = 32; blue, 0.5 mg/ml; green, 1 mg/ml. Black arrow at top of graph indicates CBZ application. E, F, Quantification of total sleep time and sleep onset latency after treatment with different CBZ concentrations, n = 32.