Figure 6.

One month after UVN, GFAP, and GAD67 immunoreactivity are differentially expressed in the vestibular nuclei of unilateral vestibular neurectomized cats according to the drug infused (NaCl, Muscimol, or gabazine). A, Illustrations of GFAP immunoreactivity in the MVN of control, UVN-NaCl, UVN-muscimol, and UVN-gabazine animals 27 d after BrdU injection performed the third postlesion day. Scale bar, 60 μm and n = 4 animals per group. B, Histograms showing the effects of the drug infusion (NaCl, muscimol, or gabazine) on the number of GFAP-immunopositive cells in the deafferented vestibular nuclei. C, Illustrations of GAD67 immunoreactivity in the MVN of control, UVN-NaCl, UVN-muscimol, and UVN-gabazine animals at D30. Scale bar, 50 μm. n = 4 animals per group. D, Histograms showing the effects of NaCl, Muscimol, or gabazine continuous infusion on the number of GAD67-immunopositive neurons in the deafferented vestibular nuclei. Only values recorded on the lesioned side are illustrated and data from both sides of control cats were pooled for direct comparison with the subgroups of vestibular deafferented cats. Different letters indicate significant differences between groups of animals: a, significantly different from NaCl group; b, significantly different from gabazine group; c, significantly different from muscimol group; and d, significantly different from control group. Analyzes were assessed by ANOVA followed by Scheffé test for all the VN and the groups (p < 0.0001; Table 3).