Table 3.
Statistical analysis of the effects of chronic intracerebroventricular (fourth ventricle) infusions of saline, GABAAR agonist (muscimol), and antagonist (gabazine) on the Ki67, BrdU, GFAP, and GAD67 immunoreactivity in the vestibular nuclei of control and unilateral vestibular neurectomized cats
| Source of variation | df | F | P* |
|---|---|---|---|
| Ki67-positive cells | |||
| Group | 2 | 3078.12 | 0.0001 |
| Vestibular nuclei | 3 | 254.65 | 0.0001 |
| Group × vestibular nuclei | 6 | 87.51 | 0.0001 |
| BrdU-positive cells | |||
| Group | 2 | 1553.91 | 0.0001 |
| Vestibular nuclei | 3 | 830.66 | 0.0001 |
| Group × vestibular nuclei | 6 | 322.53 | 0.0001 |
| Postoperative time | 1 | 25517.95 | 0.0001 |
| Group × postoperative time | 2 | 8095.05 | 0.0001 |
| Nuclei × postoperative time | 3 | 342.06 | 0.0001 |
| Group × vestibular nuclei × postoperative time | 6 | 142.66 | 0.0001 |
| GAD67-positive cells | |||
| Group | 3 | 2699.69 | 0.0001 |
| Vestibular nuclei | 3 | 2125.73 | 0.0001 |
| Group × vestibular nuclei | 9 | 344.42 | 0.0001 |
| GFAP-positive cells | 3 | ||
| Group | 3 | 2189.05 | 0.0001 |
| Vestibular nuclei | 9 | 1383.88 | 0.0001 |
| Group × vestibular nuclei | 158.22 | 0.0001 |
*Repeated-measure analysis of variance of the number of Ki67-, BrdU-, GFAP-, and GAD67-positive cells in vestibular nuclei. Group (control, unilateral vestibular neurectomized cats infused with muscimol, gabazine, or NaCl), vestibular nuclei (MVN, IVN, LVN, and SVN) and postoperative time (3 d post UVN vs 30 d post UVN) are the main fixed effects providing the sources of variation among cats. df, Degree of freedom; F, Scheffé's test; P, probability level.