Figure 1.

Effects of A_2AR stimulation on lysosomal Ca²⁺ release in healthy and NPC1 human fibroblasts. A, GPN triggers a slow monophasic and synchronous Ca²⁺ raise. B, The average amplitude and kinetic of the GPN-induced Ca²⁺ signal is not affected by thapsigargin. C, Lysosomal Ca²⁺ contents are reduced in NPC1 versus control fibroblasts (n = 3 healthy and n = 4 NPC1 fibroblast clones; 181–220 fibroblasts for each single clone). D, CGS21680 significantly increased GPN-induced signal in NPC fibroblasts, an effect abolished by ZM241385 (ZM). Data are means ± SEM. *p < 0.05 versus control; **p < 0.05 versus untreated NPC1 fibroblasts.