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. 2013 Sep 25;33(39):15388–15393. doi: 10.1523/JNEUROSCI.0558-13.2013

Figure 3.

Figure 3.

Effect of A2AR stimulation on cholesterol accumulation in the lysosomal compartment. A, Fibroblasts from healthy (CTR) or NPC (NCP1) individuals were labeled with Filipin III (blue) and anti-Lamp2 antibody (green). Bottom panels show the colocalization of two markers. Scale bar, 10 μm. B, Mean fluorescence intensity (MFI) values of Filipin III and Lamp2 in fibroblasts treated for 24 h with CGS21680 or vehicle, and Pearson's correlation coefficients (PCC) as a measure of colocalization of the two signals (PCC = 1 maximal colocalization); data are mean ± SEM from 30 to 40 cells from three clones per group. C, Filipin III fluorescence analysis of healthy (CTR) or NPC1 fibroblasts, treated for 24 h with 100 nm CGS21680, 5 μm BAPTA-AM, or their combination. *p < 0.05 versus control fibroblasts; **p < 0.05 versus untreated NPC1 fibroblasts.