Figure 2.

Neurons induce proliferative arrest and cell death of GL261 and U87 glioma cells. A, FACS dot plot gating on CFSE-labeled GL261 cells after coculture with cerebellar granule neurons. B, DNA synthesis assessed by BrdU incorporation in GL261 cells in coculture with neurons from 3 DIV culture of CGNs, or cortex, cultured primary glial cells compared with GL261 cells alone. Neurons and glial cells were cocultured with GL261 cells at 5 to 1. Data are mean ± SD from three separate experiments; ***p < 0.001. C, Representative FACS histogram with PI staining (gated on sub-G₁ phase) of GL261 cells alone and after coculturing with neurons. D, DNA synthesis by BrdU incorporation of U87 cells in coculture with neurons compared with U87 cells alone. Three DIV CGNs cocultured with U87 cells at 10 to 1. Data are mean ± SD; **p < 0.01. E, Representative FACS histogram of PI staining (gated on sub-G₁ phase) of U87 cells alone and after coculturing with 3 DIV CGNs at 10:1 (CGN to U87).