Table 2.

PD-L1 mRNA in CGNs after treatment with recombinant (rec) IFN-β

Groups	Fold expression of PD-L1	p value
Ifnb−/− + rec IFN-β/Ifnb−/−	5.773	0.001
Ifnb+/+/Ifnb−/−	1.193	0.162
Ifnb+/+ + rec IFN-β/Ifnb−/−	15.45	0.001
Ifnb+/+/Ifnb−/−>+ rec IFN-β	0.2067	0.002
Ifnb+/+ + rec IFN-β/Ifnb−/− + rec IFN-β	2.677	0.072
Ifnb+/+ + rec IFN-β/Ifnb+/+	12.95	0.001

Affymetrix gene expression analysis of PD-L1 on 3 DIV CGNs from Ifnb^+/+ or Ifnb^−/− mice, nontreated or treated with IFN-β (100 U/ml) for 3 h. RNA from triplicate CGN cultures analyzed on affymetrix mouse 430 2.0 arrays. Student's t test was used to calculate p values.