Table 1.

Antibodies used for Western blot analysis

Primary antibody	Blocking	Secondary antibody
TβRII-c16 (Santa Cruz Biotechnology) 1:200	5% non-fat dry milk	Chicken anti-rabbit coupled to horseradish peroxidase (Santa Cruz Biotechnology); 1:2000
pSmad3 (Abcam) 1:200	5% BSA	Chicken anti-rabbit coupled to horseradish peroxidase (Santa Cruz Biotechnology); 1:2000
pSmad3 (Cell Signaling Technology) 1:200	5% BSA	Chicken anti-rabbit coupled to horseradish peroxidase (Santa Cruz Biotechnology); 1:2000
Smad7 (Santa Cruz Biotechnology) 1:200	5% BSA	Chicken anti-goat coupled to horseradish peroxidase (Santa Cruz Biotechnology); 1:2000
NGF (Alomone Labs) 1:250	3% BSA	Chicken anti-rabbit coupled to horseradish peroxidase (Santa Cruz Biotechnology) 1:2000