Figure 1.

Synapsin-null mutants (syn⁹⁷) are defective for STH. A, α-Synapsin staining in adult whole-mount brain preparation of wild-type (CS) and syn⁹⁷ mutant flies. B, Histogram showing the efficiency of STH for CS and syn⁹⁷ flies to EB. Light gray bars correspond to the naive response to a 10⁻³ dilution of EB and the dark gray bars depict the corresponding behavioral response after 30 min of 5% EB exposure. C, Odorant sensitivity test for CS and syn⁹⁷ flies measured across different concentration of EB. ***p < 0.001, calculated using Student's t test with Bonferroni correction. Error bars indicate mean ± SEM, N is 9–10 sets of flies for each bar. Refer to Table 1 for raw RI values, number of sets, and p values of the experimental genotypes for EB and CO₂ habituation.