Figure 5.

LTH is a synapsin independent mechanism. A, Histogram showing the LTH responses for CS and syn⁹⁷ flies (F_(1,37) = 0.104, p = 0.749, two-way ANOVA; Turkey test for CS flies, q = 20.385, p < 0.001 and for syn⁹⁷ flies, q = 21.031, p < 0.001). Light gray and dark gray bars correspond to behavioral responses to a 10⁻³ dilution of EB, after 4 d of exposure to paraffin oil and EB, respectively. N for each bar is 9–10 sets (Table 2). B, Change in the volume of DM2, DM5, and V glomerulus, shown in light gray and black bars, respectively, for mock- and EB-exposed syn⁹⁷ flies. Raw values for glomerular volumes (μm³ ± SEM) of mock- and EB-exposed flies with number of measured glomeruli in the parentheses and statistical results [t_(df) = t value, p = p value]: for DM2 glomeruli, 2680 ± 103 (16) and 3168 ± 110 (20), t₍₃₄₎ = 3.16, p = 0.003; for DM5 glomeruli, 1086 ± 43 (16) and 1311 ± 40 (18), t₍₃₂₎ = 3.81, p < 0.001 and for V glomeruli, 4649 ± 112 (16) and 4420 ± 140 (20) with a t₍₃₄₎ = −1.23, p = 0.227. Two-way ANOVA yielded a significant difference between mock- and EB-exposed flies (F_(2,105) = 6.284, p = 0.003). Error bars indicate mean ± SEM. ***p < 0.001 and **p < 0.01 equal to 0.001 calculated using Student's t test with Bonferroni correction.