Table 1.
Showing the raw RI values* for naive and odor-exposed flies with number of sets (in parentheses) and statistical results (t values, degrees of freedom, and p values) for all the experimental genotypes
| Figure | Genotype (STH experiments) | Negative RI for EB STH |
Negative RI for CO2 STH** |
||||
|---|---|---|---|---|---|---|---|
| Naive | Exposed | t(df) = t value, p = p value | Naive | Exposed | t(df) = t value, p = p value | ||
| 1B | CS | 0.68 ± 0.009 (9) | 0.30 ± 0.02 (9) | t(16) = −13.33, p < 0.001 | 0.50 ± 0.01 (8) | 0.24 ± 0.02 (8) | t(14) = −11.70, p < 0.001 |
| syn97 | 0.71 ± 0.01 (10) | 0.71 ± 0.02 (10) | t(18) = 0.21, p = 0.83 | 0.52 ± 0.01 (10) | 0.52 ± 0.02 (10) | t(18) = 0.05, p =0.96 | |
| 2B | UAS-syn+,syn97/syn97 | 0.64 ± 0.02 (9) | 0.68 ± 0.02 (9) | t(16) = 1.53, p = 0.15 | 0.66 ± 0.02 (9) | 0.69 ± 0.03 (9) | t(16) = 0.86, p = 0.40 |
| LN1-Gal4/+; UAS-syn+,syn97/syn97 | 0.72 ± 0.02 (11) | 0.33 ± 0.01 (10) | t(19) = −18.56, p < 0.001 | 0.65 ± 0.02 (11) | 0.32 ± 0.01 (11) | t(20) = −12.32, p < 0.001 | |
| GAD1-Gal4/+; UAS-syn+,syn97/syn97 | 0.65 ± 0.02 (10) | 0.28 ± 0.02 (10) | t(18) = −11.01, p < 0.001 | 0.55 ± 0.01 (9) | 0.24 ± 0.02 (9) | t(16) = −14.59, p < 0.001 | |
| GH146-Gal4/+; UAS-syn+,syn97/syn97 | 0.78 ± 0.03 (11) | 0.74 ± 0.02 (11) | t(20) = −1.01, p = 0.32 | — | — | — | |
| mb247-Gal4,syn97/ UAS-syn+,syn97 | 0.62 ± 0.02 (10) | 0.65 ± 0.02 (10) | t(18) = 1.36, p = 0.19 | 0.66 ± 0.02 (10) | 0.70 ± 0.02 (10) | t(18) = 1.38, p = 0.18 | |
| 2C | LN1-Gal4/tub-Gal80ts; UAS-syn+,syn97/syn97—18°C | 0.73 ± 0.02 (10) | 0.67 ± 0.02 (10) | t(18) = −1.77, p = 0.09 | 0.56 ± 0.03 (9) | 0.62 ± 0.02 (9) | t(16) = 1.64, p = 0.12 |
| LN1-Gal4/tub-Gal80ts; UAS-syn+,syn97/syn97—29°C | 0.72 ± 0.02 (10) | 0.37 ± 0.05 (10) | t(18) = −6.30, p < 0.001 | 0.51 ± 0.03 (9) | 0.36 ± 0.02 (9) | t(16) = −4.50, p < 0.001 | |
| 2D | LN1-Gal4/+; tub-Gal80ts/UAS-synRNAi — 18°C | 0.69 ± 0.02 (10) | 0.30 ± 0.01 (10) | t(18) = −14.18, p < 0.001 | 0.51 ± 0.007 (3) | 0.20 ± 0.03 (3) | t(4) = −5.26, p <0.001 |
| LN1-Gal4/+; tub-Gal80ts/UAS-synRNAi — 29°C | 0.74 ± 0.05 (10) | 0.70 ± 0.03 (10) | t(18) = −0.57, p = 0.58 | 0.56 ± 0.03 (3) | 0.54 ± 0.02 (3) | t(4) = −0.19, p = 0.86 | |
| 3D | CS | 0.61 ± 0.03 (8) | 0.29 ± 0.03 (8) | t(14) = −7.11, p < 0.001 | 0.53 ± 0.03 (5) | 0.21 ± 0.04 (5) | t(8) = −6.33, p < 0.001 |
| UAS-synala-ala,syn97/syn97 | 0.68 ± 0.04 (10) | 0.64 ± 0.03 (10) | t(18) = −0.76, p = 0.46 | 0.65 ± 0.02 (10) | 0.67 ± 0.01 (10) | t(18) = 1.31, p = 0.21 | |
| LN1-Gal4/+; UAS-synala-ala,syn97/syn97 | 0.65 ± 0.02 (9) | 0.64 ± 0.01 (9) | t(16) = −0.49, p = 0.63 | 0.69 ± 0.02 (10) | 0.69 ± 0.02 (10) | t(18) = 4.44, p = 1.00 | |
| GAD1-Gal4/+; UAS-synala-ala,syn97/syn97 | 0.64 ± 0.01 (11) | 0.62 ± 0.02 (11) | t(20) = −0.63, p = 0.53 | 0.65 ± 0.01 (10) | 0.66 ± 0.02 (10) | t(18) = 0.31, p = 0.76 | |
| LN1-Gal4/+; UAS-synala-ala,syn97/+ | 0.61 ± 0.02 (10) | 0.28 ± 0.02 (10) | t(18) = −12.91, p < 0.001 | 0.49 ± 0.02 (9) | 0.18 ± 0.03 (9) | t(16) = −8.36, p < 0.001 | |
| GAD1-Gal4/+; UAS-synala-ala,syn97/+ | 0.63 ± 0.02 (10) | 0.22 ± 0.03 (10) | t(18) = −10.45, p < 0.001 | 0.60 ± 0.02 (10) | 0.28 ± 0.02 (10) | t(18) = −10.16, p < 0.001 | |
| 4B | LN1-Gal4/UAS-CaMKII-ala; tub-Gal80ts/+ — 18°C | 0.61 ± 0.02 (11) | 0.34 ± 0.02 (11) | t(20) = −8.98, p < 0.001 | 0.58 ± 0.02 (7) | 0.30 ± 0.01 (7) | t(12) = −11.37, p < 0.001 |
| LN1-Gal4/UAS-CaMKII-ala; tub-Gal80ts/+ — 29°C | 0.69 ± 0.03 (11) | 0.69 ± 0.03 (11) | t(20) = 0.10, p = 0.92 | 0.55 ± 0.03 (9) | 0.58 ± 0.03 (9) | t(16) = 0.89, p = 0.39 | |
| 4C | LN1-Gal4/UAS-CaMKIIRNAi; tub-Gal80ts/+ — 18°C | 0.65 ± 0.01 (9) | 0.27 ± 0.03 (9) | t(16) = −10.76, p < 0.001 | — | — | — |
| LN1-Gal4/UAS-CaMKIIRNAi; tub-Gal80ts/+ — 29°C | 0.62 ± 0.01 (9) | 0.63 ± 0.02 (9) | t(16) = 0.43, p = 0.67 | — | — | — | |
| Gal4 Controls (for 2B) | LN1-Gal4; syn97 | 0.70 ± 0.02 (10) | 0.64 ± 0.03 (10) | t(18) = −1.70, p = 0.11 | 0.61 ± 0.02 (10) | 0.64 ± 0.02 (10) | t(18) = 1.15, p = 0.27 |
| GAD1-Gal4; syn97 | 0.67 ± 0.03 (9) | 0.69 ± 0.01 (9) | t(16) = 0.23, p = 0.82 | 0.52 ± 0.03 (10) | 0.60 ± 0.03 (10) | t(18) = 1.97, p = 0.07 | |
| GH146-Gal4; syn97 | 0.7 ± 0.02 (9) | 0.68 ± 0.01 (9) | t(16) = −0.78, p = 0.45 | — | — | — | |
| mb247-Gal4,syn97 | 0.66 ± 0.03 (9) | 0.64 ± 0.02 (9) | t(16) = −0.63, p = 0.54 | 0.53 ± 0.02 (10) | 0.58 ± 0.03 (10) | t(18) = 1.15, p = 0.27 | |
*Values are ± SEM.
**For CO2 habituation experiments, histograms are not provided in the figures only raw RI values of before and after habituation responses are documented in the table along with number of sets and statistical results.