Table 4.
Expression of erg channel subunits in the cerebellum of P6 and 7-week-old micea
Gene | P6 mice |
W7 mice |
p | ||
---|---|---|---|---|---|
Relative expression level (%) | n | Relative expression level (%) | n | ||
erg1a + erg1a-uso | 48.4 ± 3.1 | 6 | 51.0 ± 2.6 | 6 | 0.589 |
erg1b + erg1b-uso | 2.2 ± 0.6 | 6 | 3.0 ± 0.7 | 6 | 0.699 |
erg2 | 35.3 ± 2.5 | 6 | 38.7 ± 2.5 | 6 | 0.244 |
erg3 | 14.1 ± 0.6 | 6 | 7.3 ± 0.6 | 6 | 0.002 |
aQuantitative RT-PCR: transcripts of erg subunit expression levels were normalized to the expression level of the reference gene pgk1 for P6 and adult (7-week-old) mice. The sum of all expression levels was set to 100% for both, P6 and 7-week-old mice, and the content of each gene in percentage of the total erg gene expression was calculated. Erg subunit expression levels of cerebella from P6 and 7-week-old mice (W7) were analyzed using ANOVA followed by the nonparametrical Mann–Whitney U test. Significance levels were adjusted by Bonferroni post hoc correction. Similar results were obtained with the Tfr as reference gene.