Figure 3.

The GnRH neuron projections to the median eminence receive synaptic input throughout their length. A, Camera lucida-like reconstruction of a filled GnRH neuron (red) projecting to the median eminence (ME), double-labeled with synaptobrevin 2 (VAMP2; pseudocolored cyan) antibodies to show putative synaptic inputs. a–d, Positions of single-plane confocal high-magnification. Multiple VAMP2 puncta (arrowheads) can be observed along the projection and into the ME. B, Isosurface renderings of the cell body (top) and the distal projection (bottom) showing putative synaptic inputs positive for VAMP2 labeling (cyan). C, VAMP2 pre-adsorption control.