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. 2013 Jan 2;33(1):371–383. doi: 10.1523/JNEUROSCI.0679-12.2013

Figure 2.

Figure 2.

In situ hybridization of MIP receptor in the Lymnaea CNS. A, Photomicrograph of the CGC after exposure of the CNS to the antisense probe. The in situ hybridization signals appear as pale purple dots that were aggregated around the nucleus of the cell. B, Photomicrograph of the CGC after exposure of the CNS to the sense probe. C, Photomicrograph of the B1 motor neuron after exposure of the CNS to the antisense probe. The signals were also observed around the nucleus. D, Photomicrograph of the neuropil region after exposure of the CNS to the antisense probe. Positive signals were not observed. E, F, Photomicrographs of the buccal ganglion after exposure of the CNS to the antisense probe. Identifiable neurons such as the B1, B2, and B3 motor neurons, as well as numerous other neurons, are shown to contain the MIP receptor mRNA. G, Photomicrograph of the cerebral ganglion after exposure of the CNS to the antisense probe. The CGC and other neurons contained the MIP receptor mRNA. H, Photomicrograph of the cerebral ganglion after exposure of the ganglion to the sense probe. Scale bars: A–D, 50 μm; E–H, 200 μm.