Figure 4.

SDS-PAGE for CNS factor solution and control solution secreted from the Lymnaea CNS. A, NuPAGE electrophoresis. The left lane indicates the marker (molecular mass). The middle lane indicates the data for the CNS factor solution sample that was expected to include MIPs. The right lane indicates the data for the control solution sample. The band of ∼4 kDa denoted by a red arrowhead can be observed in the CNS factor solution but not in the control solution. The control solution was prepared as the secretion of the CNSs without the cerebral ganglia that were immersed in a small amount of Lymnaea saline. This size corresponds to the B-chain of MIP II. B, Tricine SDS-PAGE. The left lane indicates the data for human insulin as a control. The middle lane indicates the data for the CNS factor solution sample that was expected to include MIPs. The right lane indicates the data for the control solution sample. Again, the band of ∼4 kDa denoted by a red arrowhead can be observed in the CNS factor solution but not in the control solution.