Verification of agonist docking results. A, In the left columns, the mutated residue is indicated and its position according to the Ballesteros-Weinstein numbering system is given. For the agonists, strychnine, parthenolide, and denatonium benzoate, threshold concentrations (TH), maximal signal amplitudes as ΔF/F values (Max ampl), and EC50 concentrations are given. X = no statistically significant value above mock-control. B, Dose–response curves of constructs challenged with different concentrations of strychnine, parthenolide, and denatonium benzoate. Changes in fluorescence after agonist stimulation (ΔF/F; y-axis) were monitored and plotted together with the corresponding agonist concentration (decadic logarithm of the agonist concentration given in μm). Plots obtained for TAS2R10 wild-type (●), TAS210L178F (▴), TAS2R10L178T (■), TAS2R46 (×), and empty vector (★) are compared within each graph.