Figure 9.

Immunocytochemical staining of TAS2R10 constructs. HEK 293T Gα16gust44 cells were transiently transfected with constructs coding for TAS2R10 and TAS2R10 mutants. The receptors (green) were visualized using an anti-HSV tag antiserum. The cell surface (red) was stained with concanavalin A. For each construct (indicated in the top left corner), a series of three images is shown: left, receptor construct; middle, cell surface; right, overlay of receptor and cell surface staining. Scale bar, 50 μm. Images were taken with a confocal laser scanning microscope.