Figure 4.

Excitatory transmission onto amygdala interneurons is preserved in il1rapl1-deficient mice. A, Amygdalar GABAergic neurons were directly visualized and recorded by GFP fluorescence after crossing il1rapl1 mutant mice with GAD67-eGFP mice (see Materials and Methods). A₁, Principal cells can be separated from interneurons by looking at cellular capacitance during the seal test. A₂, Density and capacitance of GABA-ergic (GFP⁺) and principal (GFP⁻) cells in Il1rapl1 WT and KO preparations. Number of recorded cells is indicated. B, Spiking patterns of LA interneurons. B₁, LA interneurons were classified in four subclasses based on spiking behavior (for a detailed description of interneuron classification, see Materials and Methods). B₂, Mean spiking frequency against spike number for each subclass of interneuron. C, Excitatory evoked transmission of LA interneurons after thalamic stimulation. C₁, Mean EPSC amplitude for 0.5, 1, and 5 mA stimulations in WT and KO interneurons. Calibration: 100 pA, 20 ms. C₂, Left, I/O curves of LA interneurons for a 5 mA stimulation in WT and KO interneurons. Right, Mean EPSC amplitude at 5 mA stimulation intensity for all LA interneurons. Number of recorded cells is indicated.