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. 2013 Aug 21;33(34):13805–13819. doi: 10.1523/JNEUROSCI.1457-13.2013

Figure 5.

Figure 5.

Morphology of LA excitatory synapses in constitutive il1rapl1 mutant mice. A, Morphology of LA principal cell dendrites is preserved in il1rapl1-deficient mice. A1, Portions of neurobiotin-filled LA principal cell dendrites were analyzed and compared between genotypes. A2–4, Analysis of spine density, morphology, and distribution of different spine types. B, Morphological examination of LA synaptic contacts. B1, Typical immunolabeling against PSD95. B2, Density of PSD95 clusters for both genotypes. C, Miniature EPSC recordings on LA principal cells for both genotypes. C1, Representative trace of mEPSC recordings in both genotypes. C2–3, Cumulative distribution of mEPSC frequency and amplitude for both genotypes. Insets, Medians of frequency and amplitude, respectively. D, Putative synapses were identified as closely apposed VGLUT2/PSD95 clusters (see Materials and Methods). Scale bars, 2 μm. D1, Typical immunolabeling showing apposed PSD95/VGLUT2 clusters. D2, Integrated intensity of PSD95 and VGLUT2 in apposed clusters for both genotypes. *p < 0.05. E, Paired pulse recordings for both genotypes. PPR was calculated as the ratio of the second response to the first one.