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. 2013 Jul 24;33(30):12352–12363. doi: 10.1523/JNEUROSCI.0386-13.2013

Figure 7.

Figure 7.

Synaptic overgrowth in brat mutants is rescued by decreasing the dose of mad. A–J, Confocal images of NMJ 4 double-labeled with anti-CSP (green) and anti-HRP (red). Wild-type (A), brat11/brat192 (B), tkv7/+ (C), Df(2L)162/+ (D), and dadJ1E4/+ (E) showed normal NMJ morphology. F, G, Removing one copy of wit or tkv did not suppress the synaptic overgrowth in brat mutants. The genotypes are brat11/brat192; witA12/+ (F) and brat11/brat192; tkv7/+ (G). H, I, Removing one copy of mad rescued synaptic overgrowth of brat mutants to the wild-type level. The genotypes are brat11/brat192; mad12/+ (H) and brat11/brat192; Df(2L)162/+ (I). J, Trans-heterozygous brat192/+; dadJ1E4/+ mutants showed more satellite boutons compared with wild-type. Scale bar, 10 μm. K, L, Quantification of total bouton number (K) and satellite bouton number (L) of NMJ 4 for various genotypes. Statistical significance was calculated using one-way ANOVA (n ≥ 15 NMJs; **p < 0.01; ***p < 0.001; error bars denote SEM).