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. 2013 Jul 10;33(28):11329–11345. doi: 10.1523/JNEUROSCI.5221-12.2013

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Copyright © 2013 the authors 0270-6474/13/3311329-17$15.00/0

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Figure 5. — KIF3C interacts with EB3. A, Schematic overview of full-length KIF3C and full-length KIF3C with two mutations in the SXIP motif binding site for EB3. CC, Coiled-coil. B, GFP pull-down assay was performed with HEK293T cells extracts coexpressing the indicated GFP-KIF3C fragments and mTagRFP-EB3 and analyzed by Western blotting with the indicated antibody. C, GST-EB3 and GST-EB3ΔC pull-down assays were performed with HEK293T cells coexpressing the indicated GFP-tagged KIF3C fragment and analyzed by Western blotting with the indicated antibody.