Figure 1.

Schematic representation of WT PrP, Dpl, and chimeric PrP/Dpl mutants (Chi1, Chi2, and Chi3). Sequences are hatched for PrP and white for Dpl. SP, Secretory signal peptide, cleaved during synthesis; OR, octarepeats, five repeats of eight amino acids; α 1–3 and α′1–3, α-helices 1, 2, and 3 of the globular C-terminal domain of PrP and Dpl, respectively; β1–2 and β′1–2, β sheets 1 and 2 of PrP and Dpl, respectively; GPI, sequence cleaved after attachment of the glycosylphosphatidyl inositol anchor; 3F4, epitopes recognized by the mAb 3F4 (white arrowhead). For chimeric constructs, the junctions between PrP and Dpl are indicated by a black arrowhead.